Coculture with the A2+/NY+ melanoma cell line A375 revealed consistently higher upregulation of the activation markers CD69 and CD137 as well as of the inhibitory markers lymphocyte-activation gene 3 (LAG3) and programmed cell death protein 1 (PD-1) for CD8+ A97L–T cells as compared with WT (Figure 1F and Supplemental Figure 1D). This evidence concerns the gene PDCD1 and melanoma.