To compare the ability of the SiRPα-Fc molecules to promote phagocytosis, we set up cocultures of human CD14+ monocyte-derived macrophages (MDMs) with PKH26 fluorescently prelabeled tumor cells; the cells were analyzed after 4 hours by flow cytometry (gating strategy shown in Supplemental Figure 2E). The gene discussed is SIRPA; the disease is neoplasm.