Similar to previous reported data, insulin use during pregnancy is also the significant potential associated risk factor in women with less duration of GDM onset to T2DM.20 Noctor et al. and Dunne et al. also reported that women with use of insulin during pregnancy were more prone of future progression to diabetes and/or abnormal glucose tolerance.20 We found most of the women with GDM diagnosis in second trimester and were multiparous women (occur in fourth and above pregnancy). Here, INS is linked to gestational diabetes.