CD4 and neoplasm: Previously, an innovative cryo-thermal therapy was established for primary tumor ablation, involving rapid liquid nitrogen (LN2) freezing followed by radiofrequency heating, which maximizes the release of tumor antigens and damage-associated molecular patterns (DAMPs) to establish CD4+ Th1-orchestrated tumor-specific immunity, and enhances the cytotoxicity of macrophages, CD8+ T cells, and natural killer (NK) cells, thereby eliminating micrometastases of tumors and improving long-term survival in the animal tumor models (5–8).