HE4 expression was first evaluated by immunoblotting in Beas-2B normal lung cell line and eight NSCLC lines (SK-MES-1, NCI-1703, H1975, PC-9, A549, SPC-A1, LTEP-A2, and H661) (Fig. 1A). The gene discussed is WFDC2; the disease is non-small cell lung carcinoma.