To further verify the hypothesis suggesting a regulatory axis involving circMYBL2, miR-1205, and E2F1, HCC cells were transfected with si-MYBL2/the miR-1205 inhibitor or LV-circMYBL2/the miR-1205 mimic, and E2F1 expression was analyzed by western blotting (Fig. 5F). This evidence concerns the gene E2F1 and hepatocellular carcinoma.