Firstly, we screened the top 50 genes with significant impact on the cell trajectory and found that the expression levels of THBS2, COMP and markers associated with cancer progression (e.g. POSTN, COL11A1, MMP11, MMP2, CTHRC1, etc.)progressively decreased as the pseudo-time advanced (namely pan-dCAFs and pan-iCAFs to pan-myCAFs) (Fig. 7D &Table S15). The gene discussed is MMP11; the disease is cancer.