• miR-497-5p (miR-497) significantly suppresses cell viability in AS.• miR-497 overexpression leads to reduced cell migration and tumor formation in AS and directly leads to reduced cell migration and tumor formation in AS, and can regulate VAT1, suggesting that VAT1 may promote cell migration in AS• miR-497 mimic transfection increases apoptosis in AS and hemangioendothelioma cell lines. The gene discussed is VAT1; the disease is neoplasm.