Furthermore,PACSIN1 knockout enhanced the antitumor effects of anti-PD1 therapy, as indicated by the nearly normal level of gastric epithelial rearrangement and maintenance of integrity.PACSIN1 knockout also significantly inhibited the expression of PD-1 (Figure 5C) but increased the expression of MHC-I (Figure 5D), suggesting thatPACSIN1 knockout may promote the immunotherapy of anti-PD1, inhibiting tumor growth and possibly promoting GC metastasis. This evidence concerns the gene PACSIN1 and gastric cancer.