To investigate the effect of mtDNA from CAFs on mitochondria damaged tumor cells, after H1299ρ0/A549ρ0 cells coculture with NFs-CM or iCAFs-CM, meanwhile CM was treated with DNaseI enzyme to hydrolyze mtDNA and followed by heating to inactivate enzyme [28], the OCR, SDH activity and GSH concentration were examined. This evidence concerns the gene SPIN1 and neoplasm.