To comprehensively understand the global impact of MUC5AC on the metastatic phenotype of non-small cell lung cancer (NSCLC) cells, we conducted mass spectrometry (MS) analysis on total lysates of MUC5AC-KD (A549-BrM-5AC.sh1) and scramble (A549-BrM.scr) cells. This evidence concerns the gene MUC5AC and non-small cell lung carcinoma.