RNASEL and infection: We next asked whether activation of RNase L modulates the effects of other pathways that are typically activated during infection by other dsRNA sensors.31,32 To simultaneously activate multiple pathways and test RNase L’s role, we transfected WT and RNASEL-KO A549 cells with poly(I:C), a dsRNA mimic (0.25 μg/mL), and performed RNA-seq (Figure 2A).