To understand the characteristics for the tumor cells, we performed re-clustering of CD8 T cells demonstrating nine sub-cell population including cell cycle cells (TUBA1B, HIST1H4C, KIAA0101, STMN1 and TUBB), effector cells (NKG7), effector memory cells (GZMB, ANXA1 and CREM), exhausted cells (CTLA4 and LAG3), intraepithelial cells (LIPF), macrophages cells (HSPA1A, HSPA1B and CXCL8), naïve cells (IL7R), resident memory cells (ANXA1 and GZMB) and Treg cells (IL2RA) (Fig. 7K and Figure S6B). The gene discussed is CTLA4; the disease is neoplasm.