Western blotting also showed that after silencing SENP1, the expression of anti-apoptosis protein (Bcl-2), proliferation protein (PCNA, C-MYC), and S phase checkpoint (CyclinA1 and CDK2) in AML cells was downregulated, and pro-apoptosis markers (Cleaved Caspase-3) were elevated (Fig. 2E). The gene discussed is SENP1; the disease is acute myeloid leukemia.