GPT and hepatocellular carcinoma: For addressing the possible effect exerted by AA for immuno-targeted-induced liver injury in HCC patients, we injected Hepa1-6 cells in C57BL/6 mice and treated the mice with PBS, AA, anti-PD1 + TKI, anti-PD1 + TKI + AA, respectively (Fig. 4B).We first detected the amount of AA and found that the content of AA in the anti-PD1 + TKI group was significantly lower than that in the PBS group, and the addition of AA increased the content(Fig. 4C).The results of liver enzyme showed that AST and ALT in anti-PD1 + TKI + AA group was significantly lower than that in anti-PD1 + TKI group (Fig. 4D).