We first aimed to study how different CAFs affect proliferation in spheroids (Ki-67-positive cells/spheroid area) and found that after coculture of two tongue cancer cell lines with site-matched CAFs, two CAFs affected proliferation, one of which, 0861CAF, increased proliferation in LK0412 spheroids but decreased proliferation in LK0902 spheroids. This evidence concerns the gene MKI67 and tongue cancer.