MAP1LC3B and Bloom syndrome: Moreover, mitophagy was measured via co-location of MitoTracker (a mitochondrial marker) and LC3B (an autophagosome marker) double fluorescence staining in lung tissues, and the results showed that the mitophagy is substantially reduced in BLM group while was markedly increased following TH5487 administration, which was partly reversed by additional Mdivi-1 treatment (Fig. 3E).