Moreover, we confirmed the AGR2-modulated unconventional release of 14-3-3ε and α-actinin 4 in both CMT and human breast cancer lines representing diverse molecular subtypes, including CMT-U27 and CMT-U27e (ER−/HER2+, E-cadherin+/Vimentin−), CF41.Mg and DMGT (ER+/HER2−, E-cadherin−/Vimentin+), MCF7 (ER+/HER2+, E-cadherin+/Vimentin−), and MDA-MB-231 (ER−/HER2−, E-cadherin−/Vimentin+). This evidence concerns the gene ESR1 and breast cancer.