To test the overall anti-tumor effect of MWA + 2DG compared with MWA + PBS during the memory induction phase, CD8+T cells were isolated from spleens of mice in the MWA + 2DG and MWA + PBS groups 7 days after MWA to coculture with 4T1 tumor cells in the presence of 2DG or PBS respectively (Fig. 3h). Here, CD8A is linked to neoplasm.