Additionally, we performed the apoptosis assay in vitro using Annexin V and propidium iodide (PI) staining of NSCLC cells treated with a CHK1i followed by Trx1 or TrxR1KD and found a higher occurrence of apoptosis in the Trx1 or TrxR1 KD cells treated with the CHK1i compared to either condition alone (Supplementary Fig. S3a, b, FACS gating strategies are presented in Supplementary Fig. S17). This evidence concerns the gene ANXA5 and non-small cell lung carcinoma.