Comparison of the representation of driver mutations between P0 and P3 PDX models revealed that all driver mutations that were subclonal in the primary tumor and present in P0 PDX models persisted in P3 PDX models (16/16), with two of these (a KMT2D mutation in CRUK0606 R6 and a NOTCH1 mutation in CRUK0816 R3) becoming clonally represented in P3 PDX models (Supplementary Fig. 18D). This evidence concerns the gene NOTCH1 and neoplasm.