Invasive prostate biopsy is the gold standard for diagnosing PCa, however, the positive rate of biopsy tissue is not high, and there is a risk of repeated puncture, and the detection rate of PCa is only 20% when the PSA level is between 4 and 10 ng/mL (Barcelo et al., 2019; Rodriguez Cabello et al., 2022). This evidence concerns the gene KLK3 and posterior cortical atrophy.