Moreover, after the twelve variables covering age ≤ 60 years, male, cirrhosis, chronic hepatitis B/C, elevated AFP levels, elevated CA19.9 levels, lymph node infiltration, absence/not rich vascularity, intrahepatic bile duct dilatation, splenomegaly, rim APHE and iso- or hyper-enhancement were included for multivariate logistic regression analysis. The gene discussed is AFP; the disease is chronic hepatitis B virus infection.