RNA-seq heatmap analysis and qRT-PCR validation confirmed the downregulation of c-Myc downstream molecules in ACAT1 knockdown BLCA cells, linking these molecules to cell cycle regulation and proliferation in various malignancies, including glioblastoma 52, 53, nasopharyngeal carcinoma 54, esophageal cancer 55, breast cancer 56, 57, cervical cancer 58, lung cancer 59, liver cancer 60-62, bladder cancer 63, prostate cancer 64, acute myeloid leukemia 65 and diffuse large B-cell lymphoma 66. The gene discussed is ACAT1; the disease is urinary bladder cancer.