In this study, we confirmed not only that ACAT1 promoted the proliferation and migration of BLCA cells both in vivo and in vitro but also that ACAT1 enhanced BLCA cell proliferation and metastasis via the AKT/GSK3β/c-Myc signaling pathway by modulating the cell cycle and EMT. The gene discussed is GSK3B; the disease is bladder transitional cell carcinoma.