MAP1LC3A and hydrops fetalis: Given that BECLIN-1 acts in the initiation of the autophagic process and the transcriptional gene is upregulated, in addition to ultrastructural images showing double-membrane compartments in HF-treated BMDCs, we immunostained the soluble microtubule-associated protein 1A/1B-light chain 3 (LC3) on fixed cells and analyzed them using immunofluorescence confocal microscopy to detect whether it binds tightly to autophagosome membranes upon HF stimulation.