In addition, LV-circDCAF8 downregulated the EMT-related protein E-cadherin; miR-217 mimics and NAP1L1 inhibitor also reversed the increase of N-cadherin and Vimentin (Fig. 7E, F), a quantification of the WB gels was displayed in Fig.S4A. In summary, circDCAF8 promotes HCC cell proliferation, migration, invasion and EMT by sponging miR-217 and upregulating the expression of NAP1L1. This evidence concerns the gene NAP1L1 and hepatocellular carcinoma.