In addition, western blot assay revealed the expression of NAP1L1 and EMT-related proteins N-cadherin and Vimentin was significantly increased, whereas E-cadherin expression was decreased in regorafenib resistant cells compared with those in sensitive cells, indicating that circDCAF8 may promote regorafenib resistance in HCC by regulating NAP1L1 and EMT (Fig. S5B). The gene discussed is NAP1L1; the disease is hepatocellular carcinoma.