CDH2 and hepatocellular carcinoma: In addition, western blot assay revealed the expression of NAP1L1 and EMT-related proteins N-cadherin and Vimentin was significantly increased, whereas E-cadherin expression was decreased in regorafenib resistant cells compared with those in sensitive cells, indicating that circDCAF8 may promote regorafenib resistance in HCC by regulating NAP1L1 and EMT (Fig. S5B).