The processus vaginalis sacs from four female patients with inguinal hernia did not differ statistically from the processus vaginalis sacs from thirty-three male patients with inguinal hernia with respect to presence of smooth muscles (p = 0.69), distribution of smooth muscle bundles (p = 0.146), expression of desmin (p = 0.609), smooth muscle actin (p = 0.609), presence of striated muscles (p = 0.052) and presence of myofibroblasts (p = 0.207). This evidence concerns the gene DES and Inguinal hernia.