Here, using gene expression analysis, we demonstrate unbalanced Ca2+ signaling pathways during SARS-CoV-2 (variants of concern [VOCs]: Delta, Omicron 2 and 5 EG5.1) infection in vitro using both human primary human epithelial cells from nasal brushing and HEK293T cells overexpressing ACE2 on the plasma membrane (HEK293T-ACE2). This evidence concerns the gene ACE2 and infection.