Furthermore, while the expression of PKCα total protein was demonstrated to markedly vary between tumor cell-types in culture, NDRG1 silencing resulted in the downregulation of PKCα protein levels in SK-Mel-28 melanoma cells, AsPC-1 pancreatic cancer cells (positive control), T47D breast cancer cells, Kelly and SH-SY5Y neuroblastoma cells (Fig. S2B). The gene discussed is NDRG1; the disease is melanoma.