All proinflammatory cytokines tested (IFN-γ, IL-10, IL-8, IL-12/IL-23p40, IL-2, IL-6, TNF-α, MCP-1, NGF-β, SCF, and VEGF-A) were detectable in the anal sacs of healthy dogs and AD. The gene discussed is CCL2; the disease is Alzheimer disease.