ELAVL3 and amyotrophic lateral sclerosis: Using RT-PCR and Sanger sequencing, we confirmed the existence of a 166-nucleotide sequence in mRNA transcripts, ELAVL3 CE 4a (hg38 chr19:11,463,662–11,463,496) in ALS tissues and note that this creates a frameshift leading to multiple premature stop sequences (Fig. 1c).