In light of our findings, studies on the potential roles of Pfkfb2 and Pfkfb3 isoenzymes in the differentiation of mESCs into IPCs using various tools such as CRISPR/Cas9-mediated gene inactivation are warranted, which may contribute to a better understanding of DM and lead to the development novel treatment strategies. This evidence concerns the gene PFKFB2 and diabetes mellitus.