RB1 and hepatocellular carcinoma: In human hepatocellular carcinoma (HCC), hypomethylation at CpG85 has been reported to lead to an increase in levels of an alternative RB1-E2B transcript and concomitant downregulation of the RB1 main transcript in confirmed retinoblastoma (Rb) LOI, resulting in the absence of the Rb pathway and the loss of its suppressor function (13).