HCRT and insomnia measurement: At present high-speed infrared video capture combined with computational image analysis has been used to quantitatively describe locomotor behavior and sleep posture in zebrafish larvae.3 The purpose of this study was to prove the effect of gotu kola extract (Centella asiatica) in improving the sleep activity of zebrafish larvae (Danio rerio) insomnia model, extending the total inactivity time (cumulative duration) and shortening the duration of first sleep (latency to first sleep) in light and dark phases through inhibition of orexin, ERK, p38 and Akt.