Based on the average results of WB analysis of orexin protein expression, the insomnia group has a higher average expression value of 131239 ± 7994 μg/μL compared to the normal group (122716.5 ± 5014.5 μg/μL) and different concentrations of CA extract which are respectively 2.5 μg/mL (100565 ± 9707 μg/μL), 5 μg/mL (91963 ± 9129 μg/μL) and 10 μg/mL (92655 ± 9604 μg/μL). Here, HCRT is linked to insomnia measurement.