IFNG and neoplasm: To further characterize the antitumor immune response induced by HY-oAd+9-ING-41 combination therapy, the splenocytes from tumor-bearing mice treated with PBS, 9-ING-41, 1 × 109 VPs of HY-oAd, and HY-oAd+9-ING-41 were harvested and co-cultured with irradiated MB49 cells to determine IFN-γ-secreting lymphocyte population by IFN-γ ELISpot assay.