In addition, by examining signature genes defined previously (28, 31, 32), we observed distinct functional status for each MPs subset, with the high Kupffer mod-score for C02-CD5L-Macro and C05-FCN3-Macro, high phagocytosis mod-score in the Macro clusters, similar proinflammatory mod-score in all clusters, highest M1 mod-score for C02-CD5L-Macro, and highest NASH-associated macrophages (NAMs) mod-score for C03-RNASE1-Macro (Figure 8D). Here, RNASE1 is linked to metabolic dysfunction-associated steatohepatitis.