Finally, we stratified bladder cancer patients in TCGA dataset into BHLHE41high and BHLHE41low groups based on the median expression level of BHLHE41, and performed Kaplan–Meier survival analysis, and we found that patients with lower BHLHE41 expression had worse overall survival than those with higher BHLHE41 expression (Fig. 1F). Here, BHLHE41 is linked to urinary bladder carcinoma.