Given the results on SIRT3 modulation and taking into account the debatable role played by this sirtuin in CRC carcinogenesis [37, 42, 46–48], the effects of SIRT3 silencing (siSIRT3) during iSGLT2 treatment were explored (Fig. 6 and Fig. S8). This evidence concerns the gene SIRT3 and colorectal carcinoma.