MGMT and melanoma: Through MSP, qRT-PCR, and immunoblot analysis, we confirmed that there was a correlation between the methylation status of the MGMT promoters in each of our cell lines—GBM (ACPK1 and OSU61), GSCs (GBMJ1 and NSC11), and melanoma (A375, MM415, WM852, and WM266-4)—and their MGMT mRNA and protein expression (Fig. 1).