In ELISPOT assay, in vitro co-culture of CD11c+ DCs from TDLNs and naïve CD8+ T derived from OT-I mice, IFN-γ secretion was increased in CD11c+ DCs from hsBCL9z96-treated MC38-OVA-expressing tumors compared with vehicle treatment; IFN-γ secretion was also increased in CD11c+ DCs from MC38-OVA tumor-bearing Bcl9f/fBcl9lf/f Cre-ERT2 mice compared with Bcl9f/fBcl9lf/f mice (Supplementary Fig. 5f, g). Here, ITGAX is linked to neoplasm.