HSPG2 and acute lymphoblastic leukemia: Since SOCE and IP3-mediated ER Ca2+ release are triggered by PLC activation, we treated B-ALL cells with Dex for 5 min, the time required to induce a sustained Ca2+ increase, and measured PLCγ2 activation by phospho flow cytometry in the presence of CXCR4 antagonists, CRISPR-Cxcr4 or siCXCR4.