Using these criteria, we identified LEFTY2-encoded protein (targeted by primer 15503_20) as a potential drug target in group one, HSPB1 as a potential drug target in group two, and LEFTY2-encoded protein (targeted by primer 15503_15), TNFAIP6, FGF2, ERAP1, EPHA1, and ITIH5 as potential drug targets in group three for PBC. Here, LEFTY2 is linked to primary biliary cholangitis.