In the current research, the carcinogen control group displayed EGFR, ERK, and MMP-1 overexpression, which leads to the uncontrolled cell proliferation of the cancer cells due to the induction of cell survival pathways, whereas TMZ treated group displayed a substantial decreased in EGFR, ERK, and MMP-1 proteins expression in mammary carcinoma cells. The gene discussed is EGFR; the disease is cancer.