The recent research depicts antineoplastic activity of TMZ on DMBA persuaded mammary carcinoma via the interruption of cancer cell dissemination, that triggers the apoptosis and decreases the mammary carcinoma occurrence through downstream effect of EGFR, ERK, and MMP-1 expression and the activation of apoptotic indicators p53 in rat mammary carcinoma model. This evidence concerns the gene MMP1 and breast carcinoma.