The results indicated that AC004692.4, HECW1-IT1, SFRP4, and COMP were significantly higher expressed in gallstone samples, whereas LINC01564, SLC26A3, RP1-27K12.2, and GSTA2 were markedly lower expressed in gallstone samples compared to control samples (Figure 8). The gene discussed is HECW1; the disease is gallstones.