Specifically, Pkd1 null cells generated from the Pkd1flox/-:TSLargeT mouse model of ADPKD were cultured in Matrigel and treated with KM-met, NT-met, or free metformin at a drug concentration of 300 μM and the effects on cyst growth were measured on day 8. The gene discussed is PKD1; the disease is autosomal dominant polycystic kidney disease.