Patient-derived fibroblasts from a cranioectodermal dysplasia (CED) skeletal ciliopathy patient (compound heterozygous for WDR35; c.A25-3G, p.I9TfsX7 and c.A1877G, p.E626G) were analyzed as an experimental control, as the cell line had previously been shown to exhibit shorter cilia and altered retrograde IFT trafficking when analyzed using ALPACA [30]. The gene discussed is WDR35; the disease is cranioectodermal dysplasia.