Consistent with scRNAseq data by Abdelfattah et al.34, mIF revealed that the frequency of CXCL12+ cells was highest in endothelial cells (E12), followed by pericytes (P12), Mφ/microglia (M12) and glioma cells (G12) (Fig. 3d). This evidence concerns the gene CXCL12 and glioma.