Here, we investigated the implication of APP, APP-CTFs, and Aβ on the expression and localization of mitochondrial transport components (i.e., SNPH, Miro1, TRAK1 and 2, Kif5 A, B, C isoforms and IC 1, 2) using complementary cellular and mice AD models. The gene discussed is RHOT1; the disease is Alzheimer disease.