IDUA and cervical squamous intraepithelial neoplasia: To induce CIN, we treated cells for 24 hr with the MPS1 inhibitor reversine (Hong et al., 2022; Kitajima et al., 2022; Santaguida et al., 2017; Santaguida et al., 2010), and after washout and recovery for 48 hr (Figure 1A), we quantified CIN in B16F10 cells, characterized its effects in early-stage tumors, and studied potential effects on macrophages in vitro (Figure 1A).