Of note, cells harboring normal CDK4, EGFR, and PDGFRA copy number, no mutation, and no immune or endothelial markers, which may represent normal cells such as oligodendrocytes, neurons, or astrocytes, as well as tumor cells negative for our tumor markers, were excluded from quantitative analyses and only considered in spatial analyses for accurate measurement of regional cell density. Here, CDK4 is linked to neoplasm.