We investigated the association of Rab and LC3 proteins (representing AP) by measuring the colocalization (yellow punctum) of six Rab and LC3 proteins using FITC‐conjugated anti‐LC3 antibody (green) and rhodamine‐conjugated anti‐Rab antibodies (red) in CL1‐5 (vector) and CL1‐5‐Q89L (Q89L) cells. This evidence concerns the gene AGFG1 and alkaline phosphatase measurement.